vaibhav chikne

Skills

Cell and molecular biology
Mammalian cell culture (primary & immortalized) · Stable cell line generation · Lentiviral vector design & transduction · CRISPR-Cas9 genome editing · siRNA/ASO/mRNA delivery · Molecular cloning & site-directed mutagenesis · Gene synthesis & codon optimization · Recombinant protein expression · DNA/RNA/protein isolation & quantification (biofluids, tissues, cell lines) · NGS library preparation (RNA-seq, DNA-seq, Psi-seq) · qPCR/RT-qPCR · Northern blotting · In-situ FISH · iCLIP · Polysome profiling · Epigenetic mapping · Phenotypic cell-based assays (signaling, viability, migration, proliferation) · In-vitro disease models · Microbial culture & engineering
Bioanalytical & Protein Analytics
LC-MS (Orbitrap, proteomics workflows) · Protein purification & characterization (affinity & size-exclusion chromatography, FPLC) · Protein stability assays · Immunoprecipitation & pulldown assays · Biomarker validation (ELISA, Western blot) · Flow cytometry (cell therapy QC & immunophenotyping) · Confocal microscopy & imaging biomarkers · Diagnostic assay development (PCR, qPCR, RT-qPCR, high-fidelity PCR, oligo hybridization) · Fluorescence reporter assays · Gel electrophoresis · Bioanalyzer (RNA/DNA quality control) · Mass spectrometry sample preparation & processing · In-vitro mRNA synthesis & purification · Automated workflows & liquid handling (TECAN, robotic workstations)
Data Analysis & Bioinformatics
GraphPad Prism (statistics & data visualization) · FlowJo · ImageJ/Fiji · SnapGene · PyMOL · ENSEMBL · UniProt · Primer-BLAST · BLAST · Genome Viewer · Galaxy workflows · LIMS (Labguru) · MS Office Suite · Adobe Studio
Project Leadership & Collaboration
Method Development & Validation, Analytical Transfers (TAP), Internal Audits, CAPA, Deviation Handling, Root Cause Analysis, Batch Release Analysis, Harmonization of Methods Across Labs
Project & people management · Experimental planning & execution · Cross-functional collaboration (clinicians, bioinformaticians, vendors) · Technology transfer · Mentoring & supervision of junior scientists · Laboratory operations & documentation · SOP writing & review · Budgeting · Scientific reporting & presentations · Time management · Communication & problem-solving · Recruitment · Method Descriptions & Validation
Specialized Areas
Functional Genomics, RNA/Protein biochemistry, Bioanalytical Chemistry, Laboratory Operations, Project Management, Statistical Analysis (GraphPad), Lab automation, Software & Instrument Hardware Handling, Cancer biology, Infectious diseases, Neurochemistry, RNA biology

About

• Experienced Bioanalytical Scientist with expertise in preclinical biomarker identification, assay development, and analytical validation.
• After many years in the laboratory world – from method development, validation & qualification to research management and personnel responsibility – I am seeking for the next exciting challenge where I can contribute with my analytical expertise, organizational and problem-solving skills and leadership.
• Demonstrated success in designing, executing, and statistically evaluating experiments to support application development, system validation, and workflow integration under GLP conditions.
• I have broad expertise in operating analytical laboratories with high quality and efficiency. With a logical and solution-focused approach and strong communication skills, I have had key roles that required both great scientific knowledge and business acumen.
• Specialized in protein–protein and protein–RNA interaction analysis, multi-omics, assay development, nucleic acid biochemistry, gene editing and method optimization for therapeutic discovery in preclinical models.
• I am also passionate about improvement work according to Lean and Six Sigma and have run several projects for increased efficiency spanning 5 countries, robust processes and sustainable working methods – always with quality and business benefit in focus.